Pentablock copolymer dexamethasone nanoformulations elevate MYOC: in vitro liberation, activity and safety in human trabecular meshwork cells
نویسندگان
چکیده
منابع مشابه
Dexamethasone induces specific proteins in human trabecular meshwork cells.
Previous studies have demonstrated the presence of high-affinity, glucocorticoid-specific receptors in explants of human outflow tissue and in cultured trabecular meshwork. Glucocorticoid-induced responses of scleral fibroblasts and trabecular meshwork cells were evaluated in this study. Incubation of human trabecular meshwork (HTM) and scleral fibroblast (HS) cells with 10(-7) M dexamethasone ...
متن کاملGlucocorticoid induction of the glaucoma gene MYOC in human and monkey trabecular meshwork cells and tissues.
PURPOSE To examine the intracellular and extracellular expression of myocilin in the human and primate trabecular meshwork (TM) in the presence and absence of glucocorticoids. METHODS Myocilin expression was examined in cultured human TM cells by Northern blot analysis and myocilin antibody-mediated immunoprecipitation. Myocilin expression was quantified using high-resolution two-dimensional ...
متن کاملDelayed secondary glucocorticoid responsiveness of MYOC in human trabecular meshwork cells.
PURPOSE To characterize the glucocorticoid responsiveness of the glaucoma gene MYOC (myocilin/TIGR) in cultured human trabecular meshwork (TM) cells. METHODS MYOC expression in two independently derived human TM cell lines was quantified by Western immunoblot analysis of protein levels and quantitative PCR analysis of mRNA levels. Promoter activity was measured indirectly with the luciferase ...
متن کاملAnalysis of glucocorticoid-induced MYOC expression in human trabecular meshwork cells
To understand the regulatory mechanisms governing glucocorticoid-mediated MYOC induction in human trabecular meshwork (HTM) cells, the expression and degradation of MYOC mRNA were quantified in HTM cells by Northern blot analysis, and the transcriptional activity of constructs containing variable lengths of putative MYOC promoters was assessed by luciferase reporter assay. Here, we confirmed th...
متن کاملIn vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin.
PURPOSE To determine whether trabecular meshwork-inducible glucocorticoid response/myocilin (TIGR/MYOC) protein associates with the extracellular matrix (ECM) of human trabecular meshwork (HTM) cells. METHODS The extracellular localization of TIGR/MYOC was examined by immunofluorescence microscopy in HTM cultures treated with and without dexamethasone and ascorbate and in a transformed HTM ce...
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ژورنال
عنوان ژورنال: Nanomedicine
سال: 2017
ISSN: 1743-5889,1748-6963
DOI: 10.2217/nnm-2017-0140